FAM84B expression was detected by qRT-PCR in the human glioma cell lines, U251, T98, LN-229, and U87, showing the highest expression in U87 cells, P < 0.05, as shown in Fig. 3A. In order to ensure the universality of the research results, U87 and T98 cells were taken to transfect two FAM84B siRNAs for subsequent functional experiments. Here, LRATD2 is linked to glioma.