The results from ROC analysis revealed that the optimal cut-off value of the automated UV enzymatic method for G6PD deficiency in newborns was less than 7.8 U/g Hb, which was comparable to the value reported previously [26], and for those with intermediate levels of G6PD activity, it was 7.8 to 20.8 U/g Hb. The gene discussed is G6PD; the disease is G6PD deficiency.