In our mice FDM model, we test the expression of related genes in the key pathway co-worked in myopia, astrocyte hypoxia and atropine targets, we found that Bmp6, Tlr4 and Piezo2 were all significantly changed at mRNA level (Mann–Whitney test, p = 0.0047; Mann–Whitney test, p = 0.0159; Mann–Whitney test, p = 0.0303, respectively. Here, TLR4 is linked to myopia.