KTN1 and esophageal squamous cell carcinoma: To investigate the biological function of KTN1-AS1 in ESCC cells, the overexpression plasmid of KTN1-AS1 was transfected into Kyse150 and Eca109 cells, and a striking upregulation of KTN1-AS1 was detected in the transfected cells (Fig. 3A).