In addition, we also analyzed the expression of immune checkpoint molecules in the ZS-ICC cohort and found that the mRNA expression of PD-L1/CD274 (p = 0.015), CD80 (p = 0.005), and CD86 (p = 0.045) was distinctly elevated in patients with PNI (Fig. 3h). This evidence concerns the gene CD80 and intrahepatic cholangiocarcinoma.