In silico stimulation of LANCL2 via varying doses (10−7 to 1 μΜ) of LANCL2 ligands such as ABA during the CDI model resulted in enhanced Treg populations for all days pre and post challenge with the largest changes on Days 3, 4, and 5 where the Treg recovered from 14.1%, 0.1%, and 14.6 to 43%. This evidence concerns the gene LANCL2 and clostridium difficile infection.