Next, we screened for IL‐8, a proinflammatory cytokine expressed by cancer and stromal cells,[57] promoting disease progression in pancreatic cancer[36] and other malignancies.[57b] Overall, IL‐8 was below the detection limit in BxPC‐3 monocultures and was present in the multicellular BxPC‐3 cultures, implicating potential synergistic effects and cellular crosstalk between tumor and stromal cells in our TME model. This evidence concerns the gene CXCL8 and neoplasm.