TOP2A and autism: Using adult zebrafish (2-month-old) brain tissue, we found that the embryonic treatment of flumequine and sodium salicylate induced persistent down-regulation of the 13 highest-ranking genes reported by Satterstrom et al. (8) (Satterstrom genes; Fig. 5E and table S2), indicating that Top2 inhibitor treatments also induced down-regulation of key autism risk genes, similar to what was found in the can4 homozygous mutant, and that this change in gene expression persisted to the adult stage.