H&E staining revealed that the hepatic steatosis and lipid accumulation were markedly aggravated in HFD-fed Senp1f/f;Alb-Cre mice compared with that of HFD-fed Senp1f/f mice (Fig. 7d), which was further confirmed by ORO staining and measuring hepatic concentrations of triglyceride (TG) and total cholesterol (TC) (Fig. 7e and Supplementary Fig. 9i). This evidence concerns the gene ALB and steatosis.