To prove that the endogenous ZBTB18 is also able to interact with CTBPs, co-IP followed by MS was performed in the GBM-derived brain tumor stem cell (BTSC) line, which naturally expresses ZBTB18 (BTSC268) (Figs 1C and S1E) (Masilamani et al, 2022). Here, ZBTB18 is linked to brain neoplasm.