INS and hydrops fetalis: However, after 13 weeks of HF/HS diet, HGFAC-KO mice developed glucose intolerance with a 1.6-fold increase in incremental area under the curve (iAUC, P < 0.005), as well as insulin resistance with a 30% decrease in incremental area above the curve (iAAC, P < 0.05), as measured by IP glucose and insulin tolerance tests, respectively (Figure 4, F and G).