Similar to the H358 model, we observed a gradual decrease in macrophage phagocytosis of tumor cells over time (Figure 1F and Supplemental Figure 2D) despite the continuous presence of M1 macrophages, which was confirmed by IHC with the M1 macrophage markers iNOS and TNF-α (Supplemental Figure 2E and Supplemental Figure 3C). The gene discussed is TNF; the disease is neoplasm.