In order to verify that TGF-β produced by LX2 cells can affect the TGF-β/Smad pathway in the HCC microenvironment, thereby regulating the EMT of HCC cells, we first treated the same groups of LX2 and HCCLM3 cells for 24 h and extracted cellular proteins for Western blotting analyze. Here, TGFB1 is linked to hepatocellular carcinoma.