In order to further study the impact of OTUD4 on pyroptosis and radiosensitivity in NPC cells, HONE1 and 5-8F cells with or without OTUD4 overexpression were transfected with GSDME-shRNA, and we then examined their response to ionizing radiation (Fig. 6A). Here, GSDME is linked to nasopharyngeal carcinoma.