RIC8B and obesity due to melanocortin 4 receptor deficiency: In brief, after obesity had developed in mice (at 22 weeks of age), we performed stereotactic injection of an excitatory DREADD (AAV5-hSyn-DIO-hM3D(Gq)-mCherry) into the DRN followed by treatment with CNO or saline (Fig. 2i–p).