To assess the extent to which RUNX1 regulome is conserved across B-ALL subtypes associated with distinct age groups, we examined the transcriptional program engaged upon RUNX1 inactivation by depleting RUNX1 in five cell lines, representing a range of driver mutations found in both childhood and adult ALL, namely ETV6-RUNX1 (Reh), MLL-AF4 (RS4;11), E2A-PBX1 (RCH-ACV), ETV6-PDGFRB (NALM-6) and BCR-ABL (TOM-1) (Supplementary Table 2). This evidence concerns the gene KMT2A and acute lymphoblastic leukemia.