To functionally assess the requirement for RUNX1 in leukemia, we depleted RUNX1 in vitro in four B-ALL cell lines and a control chronic myeloid leukemia (CML) cell line and monitored proliferation of leukemic cells over nine days (Supplementary Fig. 5d, red asterisks, and 7a, Supplementary Table 2). The gene discussed is RUNX1; the disease is leukemia.