Administration of rDKK1 (r-hDKK1 or r-mDKK1, 100 ng/ml) failed to increase FFA-induced steatosis of CD36 knockout hepatocytes; the ORO staining showed no significant differences either in CD36−/− HepG2 (Fig 5C) or CD36−/− AML12 cells (Fig 5D). This evidence concerns the gene DKK1 and steatosis.