To test the role of CD36 in DKK1-driven hepatic steatosis, the CD36 knockout HepG2 and AML12 cell lines were generated by the CRISPR/Cas9 editing, and the obtained knockout lines were validated by gene sequencing (Fig S2E and F) and Western blot (Fig S2G and H). The gene discussed is CD36; the disease is Hepatic steatosis.