Conventional PCR of CGG repeats of the 5′UTR and exon 1 of FMR1 gene illustrated premutation alleles with CGG repeats ranged from 55 to 200 in 34 (37%) cases of PCR product 600 bp, 2 (0.03%) intermediate cases or gray zone alleles (45–55) of PCR product 500 bp, full mutations with CGG repeats of more than 200 in 6 (0.07%) patients of PCR product greater than 700 bp and 50 (63%) normal cases amid clinical features common with those of FXS, of PCR product 450 bp compared to control group that showed normal clinical and molecular picture (Fig. 1). This evidence concerns the gene FMR1 and fragile X syndrome.