The results showed that SLC34A2, NPAS2, and NFE2 were highly positively correlated with biochemical indices of cholestasis, whereas AABR07044914.1, HPS4, POR, FMO2, ZFP354A, d-glucy, PREX2, USP2, RBM48, PDGFC, ADH6, POLG2, MCM10, LDB1, MCRIP2, TXNIP, URAD, and GPT2 were highly negatively correlated with biochemical indices of cholestasis. The gene discussed is USP2; the disease is cholestasis.