LSCM analyses of infected RF/6A cells at 24 h determined that both the ESCRT-0 component, Hgs, and the ESCRT-independent protein, ALIX, colocalize with APH0032, an A. phagocytophilum secreted effector that is expressed and localizes to the ApV membrane during late-stage infection, between 20 and 32 h (17, 30) (Fig. 4A and B). Here, HGS is linked to infection.