To investigate whether SARS-CoV-2-specific CD4+ and CD8+ T cell responses in SARS-CoV-2-unexposed donors correlate with previous CMV infection, pre-pandemic cryopreserved PBMCs from CMV seropositive (CMV+, N=28) and CMV seronegative (CMV−, N=39) healthy individuals were stimulated overnight using SARS-CoV-2 15-mer peptide pools. The gene discussed is CD4; the disease is cytomegalovirus infection.