Moreover, gene expression analysis in bulk RNA-seq showed that the majority of cuproptosis-related genes, including ATP7B, DBT, DLD, DLST, FDX1, GCSH, LIAS, PDHA1, PDHB and SLC31A1, exhibited a relative lower expression level in HCC samples compared with the normal liver samples. Here, ATP7B is linked to hepatocellular carcinoma.