The expression of 20 differentially expressed FRGs (ACSL1, ACSL4, ALOX5, ATG7, CAMKK2, CREB5, GABARAPL1, KDM6B, LAMP2, PANX2, PGD, PRDX1, PROK2, PTEN, PTGS2, SAT1, STAT3, TLR4, ULK1, and ZFP36) were further verified to study the expression changes of these FRGs in myocardial infarction tissues with time. The gene discussed is ALOX5; the disease is myocardial infarction.