In our study, it was aimed to examine the synergistic effects of Juglone and selenium on PANC-1 and BxPC-3 pancreatic cancer cells by evaluating the expression levels of CDH1, ITGB3 and COL4A3 genes and their proteins E-cadherin, β3 integrin and tumstatin, respectively, that play role in invasion and metastasis processes. This evidence concerns the gene ITGB3 and familial pancreatic carcinoma.