The general characteristics of the subjects are shown in Table 2. Most of the subjects were males (66.4%), 40-65 years old (69.4%), married (81.7%), diagnosed with DM2 (91.4%), with a history of DM that exceeds 10 years (89.2%), and using insulin treatment for DM (64.7%) (Table 2). The gene discussed is INS; the disease is diabetes mellitus.