The results revealed that a total of 9 genes were closely correlated with NAFLD and glucose metabolism, including GCK, PPP1R3C, NHLRC1, ENO3, PPP2R5D, PFKFB3, PGM2, SLC25A12, and PFKP. The gene discussed is PPP2R5D; the disease is metabolic dysfunction-associated steatotic liver disease.