A specific amplification of ~0.8 kb band for PluMV-Plu-Ind-1 and ~ 1.3 kb band for FrMV-Ind-1 was obtained in simplex RT-PCR performed with the RNA extracted from the symptomatic leaf collected from the original temple tree and two bands of the desired size were obtained in the duplex RT-PCR with the same RNA indicating mixed infection of both the viruses (Figure 6C). The gene discussed is NUBPL; the disease is infection.