Col2a1, Pax1, and Acan were among the top 30 transcripts higher in NP over FAT cells, Pax1 and Acan were in the top 30 transcripts lower in FAT over AF cells, and Col2a1was the second highest differentially expressed gene in NP over AF cells (Figure 4D). This evidence concerns the gene ACAN and atrial fibrillation.