The top transcript higher in NP over FAT cells was Glis1. The lowest transcript levels in this grouping had Slc32A1. The highest transcript levels in FAT over AF cells were noted for Glp1r, and the lowest were noted for Brinp3. Selected transcripts were validated by RISH on cells in vitro and in vivo (Figure 4E), including Klhdc7a, a transcript higher in NP cells, encoding a not well-characterized Kelch-domain containing protein and two collagen transcripts, Col9a1 and Col11a2, also higher in NP cells (Figure 4E, Figure 5 and Supplementary Table S1). This evidence concerns the gene GLIS1 and atrial fibrillation.