Therefore, we hypothesized that the abnormal expression of miRNA-190a-5p was upregulated in VCD-treated rats followed by downregulation of PHLPP1, which further activated AKT and FOXO3a proteins on AKT-FOXO3a signaling pathway, thus hyperactivation primordial follicles leading to the development of POF. Here, PHLPP1 is linked to premature menopause.