During the initiation of atherosclerosis, the generation of LAP+ Tregs (spleen: 4.4 ± 0.3% vs 2.6 ± 0.3%, respectively; P = 0.001; Figure 3) (blood: 1.7 ± 0.2% vs 1.13 ± 0.06%, respectively; P = 0.005; Figure 4) and Foxp3+ Tregs (spleen: 13.1 ± 0.5% vs 10.0 ± 0.5%, respectively; P = 0.002; Figure 3) (blood: 8.5 ± 0.3% vs 7.5 ± 0.1%, respectively; P = 0.006; Figure 4) was upregulated in the mice treated with IL-27 compared with those treated with PBS. Here, TGFB1 is linked to atherosclerosis.