When AMPK was knocked down, the level of Ser556-phosphorylated ULK1 was decreased as expected, and although the level of Ser2448-phosphorylated mTOR was increased, it was still lower than the level in the siNC control group, suggesting that some other factors may inhibit mTOR activation during ZIKV infection. The gene discussed is ULK1; the disease is Zika virus infectious disease.