NFKB1 and alkaline phosphatase measurement: Moreover, we performed RNA-seq analysis on PA-treated PEOs and found that genes upregulated upon PA treatment in FXRf/f PEOs were primarily related to the NLRP3 inflammasome, mitophagy, and noncanonical NF-κB signaling (Figure S3, a-f), which are well-recognized molecular cascades implicated in the pathogenesis of AP.