Our results showed that GTPBP4 protein was highly expressed in NSCLC tissues and lowly expressed in the corresponding paracancerous tissues of NSCLC; GTPBP4 highly expressed in lung cancer cells were expressed in the cytoplasm and nucleus (Figure 2(a)); the difference between the two groups was statistically significant (t = 5.214; P=0.019; Figure 2(b)). The gene discussed is GTPBP4; the disease is lung cancer.