We used RNA-seq data from the TCGA datasets, along with statistical and bioinformatics methods, such as analysis of differentially expressed genes (DEG), Kaplan-Meier (KM) survival analysis, Cox and logistic regression analysis, and single-sample gene set enrichment analysis (ssGSEA), to better understand the roles that PTK6 played in the progression of BC. The gene discussed is PTK6; the disease is breast cancer.