CD68 and Anorexia: In our hands, minocycline pretreatment dramatically reduced CD68 expression within the DVC and hypothalamus and worsened DON-induced anorexia and neuronal activation, as we previously observed after microglia deletion by PLX3397. Minocycline, like most antibiotics, has off-targets such as modulation of iron chelation, oxidative stress or leucocyte functions [88, 89], which nevertheless must be kept in mind as interference on the results obtained, even if unlikely.