PIK3CA inhibitors BKM-120 and Wortmannin showed significant inhibitory effects at higher concentrations on all cell lines, while LY294002 at higher dose had significant effect on all cell lines but RBE (ICC cell line), EGI1 (ECC cell line), and HEPG2 (hepatoblastoma cell line). This evidence concerns the gene PIK3CA and intrahepatic cholangiocarcinoma.