Since tumor suppressors of the PIK3 pathway such as PTEN (phosphatase and tensin homolog) were found to be mutated in combined HCC/ICC (Cosmic, 2021), we tested four different PI3K inhibitors, three of them inhibit predominantly PIK3CA (BKM120, Wortmannin, and LY294002) and one PIK3CD (CAL-101). Here, PTEN is linked to intrahepatic cholangiocarcinoma.