In patients where IHC analysis showed tumour cell positivity for FRα (≥5% tumour cells in the sample), the concentration of sFRα and proportion of samples with detectable levels, were significantly greater (7.6 ± 1.8 ng/ml; detectable in 50%), compared to patients with FRα-negative tumours (2.6 ± 1.2 ng/ml; detectable in 21%) (Fig. 4a, left). The gene discussed is FOLR1; the disease is neoplasm.