We confirmed this result by western blotting for phosphorylated γH2AX, which similarly showed an increase in the ratio of phosphorylated to total γH2AX following knockdown of SFXN4 in COV362 HGSOC cells as well as in FTTovarian cancer stem cells and FTT CisR cisplatin-resistant ovarian cancer stem cells (Fig. 3G). This evidence concerns the gene SFXN4 and cancer.