Immunohistochemical staining showed that the MMP-9 level increased in the spinal instability group, while MA inhibited the MMP-9 level in the spinal instability + MA group (Fig. 7C, D), also, the Collagen II level was decreased in the spinal instability group, while the Collagen II level was increased in the MA group (Fig. 7C, D). The gene discussed is MMP9; the disease is spinal stenosis.