TLR4 was enriched in a variety of important signaling pathways, such as alkylation metabolism, cell metabolism regulation, cell negative regulation metabolism, cell cycle complex metabolism regulation, etc. KEGG enrichment analysis showed that the pathways of transcription regulation, ER stress, oxidative phosphorylation, MAPK signaling pathway, EBV infection, and cell adhesion molecules were influenced (Fig. 5F). The gene discussed is TLR4; the disease is Epstein-Barr virus infection.