In the present study, we aim to analyze the expression of EZH2, FOXM1 and CAFs markers (α-SMA, FSP1 and FAP) in GC tissue by using mIF, and tried to analyze their correlation, the correlation between FOXM1 or EZH2 positive GC cells with CAFs, and their correlation with clinicopathological parameters and prognosis of GC. This evidence concerns the gene ACTA1 and gastric cancer.