To thoroughly understand the role of circ_AFF4 and IGF2BP3 in BM-MSC osteogenic, we firstly transfected BM-MSCs with lentiviruses harboring circ_AFF4 or shIGF2BP3 for a stable overexpression or knockdown followed by and osteogenesis capacity evaluation after culturing in OM for 14 days. This evidence concerns the gene IGF2BP3 and ocular melanoma.