Furthermore, as assessed by RT-qPCR (Fig. 2D) and western blot (Fig. 2E), the osteogenic differentiation factors, including ALP, Runx2, OPN and Colla1, were reduced in circ_AFF4-depleted BM-MSCs after 14-day culture in OM in relation to the non-treated group and the shNC group. This evidence concerns the gene SPP1 and ocular melanoma.