After the successful construction of HIBI model, we found that from normal rats to HIBI rats and then to NEC + HIBI rats, the mortality of animals increased, accompanied by worsening brain and intestinal tissue damage, as well as increased CXCL1/CXCR2 expression levels, suggesting the occurrence of inflammation in intestinal and brain tissues. This evidence concerns the gene CXCR2 and necrotizing enterocolitis.