The results suggested that in the intestinal tissues (Fig. 2A), the relative expression of CXCL1 was significantly increased in NEC rats 3 (1.27 ± 0.18 vs. 1) and 72 (1.58 ± 0.19 vs. 0.93 ± 0.09) h after intragastric administration compared to the control groups (P < 0.05). This evidence concerns the gene CXCL1 and necrotizing enterocolitis.