Similarly, genes encoding circadian clock regulators (Dbp, Nr1d1 and Nr1d2), KLF transcription factors (Klf2 and Klf6) and cell signaling molecules (Rhob and Shank3) were also upregulated in sustained obesity (cohort 2) but not in the reversion group (cohort 3; Fig. 4e and Extended Data Fig. 9f). This evidence concerns the gene CLOCK and obesity due to melanocortin 4 receptor deficiency.