Next, BAT transplantation experiments were performed following thedesign shown in Extended Data Fig. 3h.The results demonstrated that BAT transplantation from WT donors in KOrecipients ameliorates endothelial dysfunction, reduces endothelial cellapoptosis, improves endothelial integrity, inhibits inflammation (TNF-α,IL-1β and IL-6) or adhesion molecule (VCAM-1, ICAM-1 and E-selectin)responses in MAECs (Extended Data Fig. 4a–e). This evidence concerns the gene TNF and endothelial dysfunction.