MC38 murine or luciferase-tagged HCT-116 human colon tumor cells were seeded into the liver by intrasplenic injection and, one day later, treated with vehicle or m830 anti-TEM8 antibodies (Fig. 7e–h and Supplementary Fig. 16a).m830 significantly reduced liver tumor burden and improved overall survival in both models, suggesting that TEM8 antibodies may aid in the treatment of established metastatic disease. This evidence concerns the gene ANTXR1 and metastatic neoplasm.