Using western blotting (Fig. 2g), we observed that linc00976 knockdown decreased protein levels of SLC7A11, SLC40A1, and GPX4, and increased those of cyclooxygenase 2 (COX2) and transferrin, suggesting that linc00976 might regulate ferroptosis-associated genes to affect CCA development. This evidence concerns the gene CCDC26 and cholangiocarcinoma.