MRC2 and sarcoma: To examine the efficacy of A5/158-vc-MMAE in vitro, increasing concentrations of A5/158-vc-MMAE and Isotype-vc-MMAE were incubated with Endo180-positive sarcoma (MG-63, HT-1080, and A-204), Endo180-low sarcoma (G-402 and SK-UT-1), and Endo180-negative (MCF-7 and HT-29) control cell lines (Fig. 4B).