However, the exosomal nature of extracellular vesicles isolated from porcine cells and the supernatant of a canine sarcoma cell line (K9STS) was shown by the typical size distribution in the range of 50 to 100 nm, as measured by dynamic light scattering (Fig. 4c, upper panel), and by the presence of Hsp70 and other typical exosomal markers such as CD9 and CD63 on the surface of the extracellular microvesicles, as determined by flow cytometry (porcine cells: Fig. 4c, middle panel; canine tumor cells: Fig. 4c, lower panel). This evidence concerns the gene HSPA1A and sarcoma.