Ding et al. used cell culture and mouse models to show that treatment with isolated EVs of PKD1-cells and urinary EVs from autosomal dominant polycystic kidney disease (ADPKD) patients which were extracted using UC and filtration methods as well as characterized via WB analyses activated the proliferation and formation of cyst-like structures. Here, PKD1 is linked to autosomal dominant polycystic kidney disease.